abstract：:The location of lipopolysaccharide (LPS) was studied by immunofluorescence and immunoelectron microscopy in macrophages infected with a non-invasive Shigella dysenteriae 1 strain. Bacterial degradation began only 3 h after the end of infection. The first visible sign of degradation was detected by immunogold labelling...

journal_title：Biology of the cell

authors： Dacosta B,Ryter A,Mounier J,Sansonetti P

更新日期：1990-01-01 00:00:00

abstract：:The ultrastructure of the Drosophila subobscura chromosome regions around the breakpoints of the complex E1 + 2 + 9 + 12 gene arrangement was analyzed. This overlapping inversion is formed by the association of the E1, E2, E9, and E12 simple inversions. Ultrastructure of sections involving 58D/59A, 61C/D, 62D/63A, 64B...

journal_title：Biology of the cell

authors： Cuenca JB,Saura AO,Sorsa V,de Frutos R

更新日期：1990-01-01 00:00:00

abstract：:Using gold labelled neoglycoproteins containing either alpha-D-glucose, N-acetyl-beta-D-glucosamine, alpha-D-mannose, 6-phospho-alpha-D-mannose, and alpha-L-fucose (BSA), we investigated their intranuclear binding sites in the TG human cell line. Although gold-labelled BSA did not give any noticeable labelling, the pr...

journal_title：Biology of the cell

authors： Bourgeois CA,Monsigny M

更新日期：1990-01-01 00:00:00

abstract：:The ultrastructural morphology of Pneumocystis carinii obtained from nonimmunosuppressed rabbit is described in details. Golgi complex and primary lysosomes of P carinii are described here for the first time. They are easily revealed by the zinc iodide-osmium tetroxide cytochemical reagent. Thiamine pyrophosphatase an...

journal_title：Biology of the cell

authors： Palluault F,Dei-Cas E,Slomianny C,Soulez B,Camus D

更新日期：1990-01-01 00:00:00

abstract：:The first step of polyamine uptake is the binding of polyamines to the cell membrane. In order to characterize the specificity of the putrescine binding sites at the surface of the glioblastoma cells (U251), we have carried out competition experiments between putrescine bound to latex microspheres and vizualized by sc...

journal_title：Biology of the cell

authors： Quemener V,Moulinoux JP,Khan NA,Seiler N

更新日期：1990-01-01 00:00:00

abstract：:In cold-stressed oocytes of Pleurodeles waltl, lampbrush chromosome lateral loops exhibited important structural modifications which were visualized under light microscopy. Electron microscopy study revealed that the RNP particles associated with growing transcripts in the matrix of these loops were 15 nm at 8 degrees...

journal_title：Biology of the cell

authors： N'Da E,Bonnanfant-Jais ML,Moreau N,Angelier N

更新日期：1990-01-01 00:00:00

abstract：:In spite of the constant development concerning physical mapping of eukaryotic genomes, the mouse chromosome 19 remains poorly characterized. In order to improve the possibilities for studying this chromosome, we have constructed a chromosome-specific EcoRI DNA fragment library from mouse chromosomes 19 sorted by flow...

journal_title：Biology of the cell

authors： Baron B,Métézeau P,Kiefer-Gachelin H,Goldberg ME

更新日期：1990-01-01 00:00:00

abstract：:Image analysis was used to study the repair process of a circular mechanical lesion of confluent human endothelial cells in culture after irradiation (10 Gy) prior to wounding. Coverage of denuded areas 48 and 96 h after injury of endothelial cells was identical in control and irradiated cultures, although the labelin...

journal_title：Biology of the cell

authors： Klein-Soyer C,Beretz A,Cazenave JP,Driot F,Maffrand JP

更新日期：1990-01-01 00:00:00

abstract：:HT 29-D4 is a clonal cell line, derived from the human colon adenocarcinoma cell line HT 29, which can be induced to differentiate into enterocyte-like cells by replacing glucose with galactose in the culture medium (Fantini et al. [1986], J. Cell Sci. 83, 235-249). Both undifferentiated and differentiated HT 29-D4 ce...

journal_title：Biology of the cell

authors： Fantini J,Verrier B,Marvaldi J,Mauchamp J

更新日期：1989-01-01 00:00:00

abstract：:The chromosomal localization of the mouse gene coding for the 68 kDa intermediate filament subunit of neurones (NF-L) was determined by in situ hybridization using specific 3H-labelled DNA probes. There is only one copy of the NF-L gene. The gene encoding NF-L is located on chromosome 14 region (D1-E1). ...

journal_title：Biology of the cell

authors： Mattei MG,Duprey P,Li ZL,Mattei JF,Paulin D

更新日期：1989-01-01 00:00:00

abstract：:Immunogold labeling and biochemical methods were used to localize the site of viral protein synthesis in frog virus 3 (FV3)-infected baby hamster kidney (BHK) cells. Immunogold labeling studies of Triton-extracted (cytoskeletons), FV3-infected BHK cells with antivimentin antibodies showed that the major components of ...

journal_title：Biology of the cell

authors： Murti K,Goorha R

更新日期：1989-01-01 00:00:00

abstract：:Detergent permeabilized Euplotes eurystomus (a fresh water hypotrichous ciliate) was reacted with monoclonal and polyclonal antibodies specific for either detyrosinated or tyrosinated alpha-tubulin (Glu- or Tyr-tubulin). The isolated cytoskeleton-nuclear complex was examined by Western immunoblotting and by immunofluo...

journal_title：Biology of the cell

authors： Olins DE,Olins AL,Robert-Nicoud M,Jovin TM,Wehland J,Weber K

更新日期：1989-01-01 00:00:00

abstract：:A new class of fluorescent dye which upon excitation at 488 nm turns red is shown to be probe-suitable for using in flow cytometry alone or in conjunction with fluorescein derivatives. 7-dimethylamino 3-(p-formylstyryl) 1,4 benzoxazin 2-one is suitable for rendering microorganisms, such as Plasmodium merozoites and ce...

journal_title：Biology of the cell

authors： Monsigny M,Midoux P,Le Bris MT,Roche AC,Valeur B

更新日期：1989-01-01 00:00:00

abstract：:As a rule, chloride movement (JC1-) across amphibian skin is considered to be passive; this is implied in fact for preparations incubated in Ringer's fluid, since short-circuit current (Isc) is the quantitative expression of net, active sodium transport (JNa+). The nature of the Cl- pathway(s) was investigated by incu...

journal_title：Biology of the cell

authors： Crabbe J,Beaujean V,Devuyst O

更新日期：1989-01-01 00:00:00

abstract：:During wound-healing in cultured frog skin fragments, fibronectin (FN) was detected in the dermal-epidermal junction. Intracellular fibronectin was stained using permeabilization and DAB immunoperoxidase. With electron microscopy intracytoplasmic FN granules were localized in the epidermal processes of the stratum ger...

journal_title：Biology of the cell

authors： Denefle JP,Zhu QL,Lechaire JP

更新日期：1989-01-01 00:00:00

abstract：:This study reports the synthesis of l-desamino-7-lysine- (fluorescein)-8-arginine-vasotocin (7-lys(flu) dAVT), and describes its biological activity in the isolated urinary bladder of the toad Bufo marinus. 7-lys(flu)dAVT was fully active in increasing bladder permeability to water. A half-maximal hydroosmotic respons...

journal_title：Biology of the cell

authors： Eggena P,Buku A

更新日期：1989-01-01 00:00:00

abstract：:This study was undertaken to evaluate whether hypertrophy and hyperplasia of the pancreatic acinar cells induced by caerulein remained after termination of the hormonal treatment. Rats received subcutaneous injections of saline or caerulein for 4 days and were killed immediately after termination of treatment or 2, 15...

journal_title：Biology of the cell

authors： Morisset J,Grondin G

更新日期：1989-01-01 00:00:00

abstract：:This study was undertaken to evaluate the effects of caerulein, a CCK analog, on the different cell populations of the pancreatic tissue and their respective turnover. Rats received saline or caerulein subcutaneously and 3H-thymidine intraperitoneally three times a day for 4 days. They were sacrificed immediately afte...

journal_title：Biology of the cell

authors： Morisset J,Grondin G

更新日期：1989-01-01 00:00:00

abstract：:The membrane content in phospholipids along the secretory pathway in rat pancreatic B-cells was studied in situ by high-resolution cytochemistry, applying the recently introduced phospholipase A2-gold technique. The gold particles were mostly associated with cell membranes, and the various types of membranes were labe...

journal_title：Biology of the cell

authors： Coulombe PA,Bendayan M

更新日期：1989-01-01 00:00:00

abstract：:Antidiuretic hormone induces, in the apical plasma membrane of amphibian urinary bladder epithelial cells, the exocytotic insertion of intramembranous particle aggregates that probably contain water channels. Purification of the apical membrane is a way to characterize the aggregates. The isolation of such purified me...

journal_title：Biology of the cell

authors： Tacnet F,Chevalier J,Coudrier E,Berthonaud V,Ripoche P

更新日期：1989-01-01 00:00:00

abstract：:Effect of estradiol-17 beta on rat vaginal epithelial cells (VEC) was studied by transmission electron microscopy. During the normal estrous cycle in adult rats keratinization and exfoliation of VEC was observed only in the estrus phase, though tonofilament bundles were seen distributed in the cytoplasm of the VEC dur...

journal_title：Biology of the cell

authors： Gupta PD,Vijayasaradhi S,Reddy AG

更新日期：1989-01-01 00:00:00

abstract：:The water permeability of collecting ducts is greatly increased by the antidiuretic hormone, vasopressin (VP). Freeze-fracture studies were carried out to test if this permeability increase is associated with the appearance of intramembrane particle (IMP) aggregates and whether increased doses of VP lead to an increas...

journal_title：Biology of the cell

authors： Kubat B,Lorenzen M,Reale E

更新日期：1989-01-01 00:00:00

abstract：:Toad bladder epithelial cells were homogenized and fractionated by Percoll density-gradient centrifugation. Binding of tritium-labeled vasopressin ([3H]AVP) was measured in surface membranes (SM), microsomes (M), and a 100,000 g (60-min) microsomal supernatant fraction (S). More than two-thirds of the total receptors ...

journal_title：Biology of the cell

authors： Eggena P,Ma CL

更新日期：1989-01-01 00:00:00

abstract：:Detergent extraction of human blood platelets pre-treated with Taxol to stabilize microtubules allows isolation of marginal band (MB) cytoskeletons. We studied MB cytoskeleton structure using dark-field light microscopy and negative stain electron microscopy (EM). Dark-field illumination clearly demonstrated the "hoop...

journal_title：Biology of the cell

authors： Kowit JD,Linck RW,Kenney DM

更新日期：1988-01-01 00:00:00

abstract：:Alpha 2-macroglobulin (alpha 2M) is a plasma inhibitor of proteinases, the steric mechanism of which is based on a considerable conformational change. The typical and distinct H-like shape of alpha 2M-chymotrypsin (alpha 2M-chy) complexes seen by electron microscopy led us to an ultrastructural study of the binding of...

journal_title：Biology of the cell

authors： Boisset N,Taveau JC,Barray M,Van Leuven F,Delain E,Lamy JN

更新日期：1988-01-01 00:00:00

abstract：:The p126 protein is synthesized by P. falciparum between the 32nd and the 36th hour of the erythrocytic cycle, and is localized in the parasitophorous vacuole. It is processed when schizonts rupture and the major fragments (50, 47 and 18 kDa), which are released into culture supernatant, have been characterized using ...

journal_title：Biology of the cell

authors： Delplace P,Bhatia A,Cagnard M,Camus D,Colombet G,Debrabant A,Dubremetz JF,Dubreuil N,Prensier G,Fortier B

更新日期：1988-01-01 00:00:00

abstract：:Variant surface glycoprotein (VSG) of Trypanosoma brucei brucei AnTat 1.1 was released by means of the procedure described by Baltz et al. ([1976], Ann. Immunol. [Inst. Pasteur] 127C, 761-774). The concanavalin-A chromatography yielded 3 VSG fractions according to the addition, in the elution buffer, of alpha-methyl-D...

journal_title：Biology of the cell

authors： Boutignon F,Hublart M,Gomes V,Mendonca-Previato L,Tetaert D,Huet-Duvillier G,Degand P

更新日期：1988-01-01 00:00:00

abstract：:Revision of the reference map of the polytene chromosomes of Drosophila subobscura was started by means of the electron microscope. We present a map of regions 70B to 72D of the E chromosome obtained from squashed and thin-sectioned salivary glands. It was observed that the total number of bands in divisions 70B to 72...

journal_title：Biology of the cell

authors： Pérez-Alonso M,Cuenca JB,De Frutos R

更新日期：1988-01-01 00:00:00

abstract：:Photoperiodic control of several biological rhythms is exerted through the inhibitory effect of light on melatonin synthesis in the pineal organ. Hydroxyindole-O-methyltransferase (HIO-MT), the last acting-enzyme in melatonin biosynthesis, constitutes a specific marker of melatoninergic cells. In the present study, an...

journal_title：Biology of the cell

authors： Guerlotte J,Voisin P,Brisson P,Faure JP,Collin JP

更新日期：1988-01-01 00:00:00

abstract：:The development of a single, very large (25-35 microns diameter) nucleolus during oogenesis in the crustacean Acanthocyclops vernalis is described. The nucleolus is the site of ribosomal RNA production in the egg, as shown by in situ hybridization, and apparently the only source, as accessory cells are not observed. R...

journal_title：Biology of the cell

authors： Standiford DM

更新日期：1988-01-01 00:00:00

abstract：:We studied the behaviour in interphase and mitotic human cells of a 125 kDa (pI 6.5) antigen, associated with the nuclear matrix and detected in proliferating cells. Indirect immunofluorescence with a specific monoclonal antibody reveals that during interphase in WISH and Namalwa cells, as well as phytohaemagglutinin-...

journal_title：Biology of the cell

authors： Todorov IT,Philipova PN,Zhelev NZ,Hadjiolov AA

更新日期：1988-01-01 00:00:00

abstract：:Several transformed cells have been demonstrated to secrete growth factors. We studied the effect of conditioned medium from neoplastic rat pleural mesothelial cells on normal and neoplastic mesothelial cell growth. The results showed that the concentrated conditioned medium stimulated neoplastic mesothelial cell grow...

journal_title：Biology of the cell

authors： Van der Meeren A,Levy F,Bignon J,Jaurand MC

更新日期：1988-01-01 00:00:00

abstract：:Cell nuclei may contain significant quantities of the metals Fe, Ni, Cu, Zn, since they are present in the nucleo-enzymes and/or nucleic acids. These metals have been detected by X-ray microanalysis in situ in dinoflagellates (Kearns et al). Aluminum was only detected in cell nuclei in cases of natural or provoked int...

journal_title：Biology of the cell

authors： Quintana C,Olmedilla A,Antoine N,Ollacarizqueta A

更新日期：1987-01-01 00:00:00

abstract：:We studied mechanisms that mediate recognition of human erythrocytes (HRBC) and sheep erythrocytes (SRBC) by rat liver macrophages. We used an in vitro cell binding assay that allows spontaneous formation of cell contacts. Binding of HRBC to rat macrophages shows the following characteristics: inhibition studies with ...

journal_title：Biology of the cell

authors： Mohr M,Kolb H,Kolb-Bachofen V,Schlepper-Schäfer J

更新日期：1987-01-01 00:00:00

abstract：:Primary cultures of cells from breast carcinomas were attempted in 74 cases. Growth was observed in 46 cases. Using immunochemical demonstration of keratin proteins (KER), epithelial membrane antigen (EMA) and carcinoembryonic antigen (CEA), three morphologically distinct cell populations were characterized and descri...

journal_title：Biology of the cell

authors： Muller D,Fricker JP,Millon-Collard R,Abecassis J,Pusel J,Eber M,Methlin G

更新日期：1987-01-01 00:00:00

abstract：:We have identified and characterized a membrane glycoprotein located at the apical plasma membrane of adult human colon epithelial cells, by the use of the monoclonal antibody technique in combination with immunocytochemical and biochemical methods. Analysis of membranes extracted with Triton X-114 and treated with sp...

journal_title：Biology of the cell

authors： Le Bivic A,Hirn M,Reggio H

更新日期：1987-01-01 00:00:00

abstract：:A monoclonal antibody of the IgG2a subclass was isolated from the supernate of a hybridoma line obtained with splenocytes from a mouse immunized with a crude nucleolar fraction of human Namalwa cells. This antibody identifies a single nuclear polypeptide antigen characterized by: (a) presence in proliferating human ce...

journal_title：Biology of the cell

authors： Philipova RN,Zhelev NZ,Todorov IT,Hadjiolov AA

更新日期：1987-01-01 00:00:00

abstract：:Rotation-mediated aggregate cultures of foetal rat liver cells were prepared and grown in a chemically defined medium. Their capacity for cellular organisation and maturation was studied over a culture period of 3 wk by using both morphologic and biochemical criteria. It was found that within each aggregate, distinct ...

journal_title：Biology of the cell

authors： Guigoz Y,Werffeli P,Favre D,Juillerat M,Wellinger R,Honegger P

更新日期：1987-01-01 00:00:00

abstract：:The dermis of the frog skin (Rana esculenta) displayed a remarkable organization of vertical and horizontal tracts. Vertical thick tracts connected the dermal Stratum spongiosum with the subcutaneous tissue. Horizontal thin tracts were found alongside and contiguous to them. The thick tracts were sheathed by collagen ...

journal_title：Biology of the cell

authors： Denèfle JP,Zhu QL,Lechaire JP

更新日期：1987-01-01 00:00:00

abstract：:Porcine lutropin (pLH) and human choriogonadotropin (hCG) recognize the same hormonal receptor and elicit the same steroidogenic response in porcine Leydig cells in primary culture. We compared the variation in the number of occupied and free receptors present on the cell surface under short-term stimulation by the tw...

journal_title：Biology of the cell

authors： Genty N,Salesse R,Garnier J

更新日期：1987-01-01 00:00:00